Characterization of a satellite RNA associated with strain K8 of cucumber mosaic virus.
نویسندگان
چکیده
Cucumber mosaic virus strain K8 (K8-CMV), originally isolated in NW Italy from Cucumis metuliferus, was sent to us by Dr. Lisa after being passaged and multiplied in tobacco. Northern blots to probes against Fny-CMV (subgroup I) and Ls-CMV (subgroup II) showed K8-CMV to belong to subgroup I of CMV isolates (1). In agarose gel electrophoresis of virion encapsidated RNAs a fifth RNA was found, shown to be a satellite RNA (K8-sat RNA). K8-sat RNA was purified from polyacrylamide gels, and its 5 'capped, 339 nucleotide (nt) long sequence (Fig. 1) was determined by both partial enzymic degradation of 3' or 5'-end labelled RNA, and by dideoxy-chain termination on the RNA template using primers complementary to nt 166-177 and to nt 331-339. Sequence homology between K8-sat RNA and other reported CMV-sat RNAs of similar size, ranged from 85% to 94%. The biology of K8-sat RNA was characterized: it attenuated the symptoms induced in tobacco and tomato by Fny-CMV and Ls-CMV, and had no effect on the symptoms induced in squash. Also, K8-sat RNA accumulated in solanaceous hosts to much higher levels than in cucurbits. These biological properties have also been reported for other well-characterized CMV-sat RNAs,
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عنوان ژورنال:
- Nucleic acids research
دوره 18 15 شماره
صفحات -
تاریخ انتشار 1990